A miniaturized test for stress biomarker detection by quantum dots on microarrays

verfasst von

Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, Carsten Zeilinger

Abstract

We aim to develop a benchtop imaging device to quantitatively measure stress biomarkers like HSP90 on a biochip. Measurement of HSP90 is based on the masking of fluorescence of Streptavidin conjugated Quantum dots (Sav-QDs) when HSP90 attaches to it. The higher the masking of fluorescence, the higher the HSP90 concentration. Measurement of fluorescence is done by a custom-built optical device. The goal in this work was to demonstrate a testing system which is handy to use in the field, cheaper and with a simplified readout system for the users. Sav-QDs are fixed on nitrocellulose coated glass slides (NC slides). A biotinylated antibody is attached to Sav-QD. When HSP90 interacts with the antibody it causes masking of fluorescence, which is dependent on the concentration of HSP90 in serum. The imaging device developed for measurement is based on a CMOS sensor. It uses narrow bandpass filters, optically eliminating fluorescence produced by the background. Analysis of the results shows that these agree well with those of standard laboratory equipment using a photomultiplier tube (PMT) scanner to detect HSP90 in the nanomolar range. Results from the work show that the used approach is promising for developing a multifunctional, robust, and point-of-care detection system for stress biomarkers.

Organisationseinheit(en)

PhoenixD: Simulation, Fabrikation und Anwendung optischer Systeme
Hannoversches Zentrum für Optische Technologien (HOT)
Zentrum für Biomolekulare Wirkstoffe (BMWZ)

Typ

Aufsatz in Konferenzband

Anzahl der Seiten

9

Publikationsdatum

27.03.2024

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Elektronische, optische und magnetische Materialien, Atom- und Molekularphysik sowie Optik, Biomaterialien, Radiologie, Nuklearmedizin und Bildgebung

Elektronische Version(en)

https://doi.org/10.1117/12.3002242 (Zugang: Geschlossen)