A miniaturized test for stress biomarker detection by quantum dots on microarrays

authored by
Anusha Kishore, Arun Mathew Varughese, Bernhard Roth, Carsten Zeilinger

We aim to develop a benchtop imaging device to quantitatively measure stress biomarkers like HSP90 on a biochip. Measurement of HSP90 is based on the masking of fluorescence of Streptavidin conjugated Quantum dots (Sav-QDs) when HSP90 attaches to it. The higher the masking of fluorescence, the higher the HSP90 concentration. Measurement of fluorescence is done by a custom-built optical device. The goal in this work was to demonstrate a testing system which is handy to use in the field, cheaper and with a simplified readout system for the users. Sav-QDs are fixed on nitrocellulose coated glass slides (NC slides). A biotinylated antibody is attached to Sav-QD. When HSP90 interacts with the antibody it causes masking of fluorescence, which is dependent on the concentration of HSP90 in serum. The imaging device developed for measurement is based on a CMOS sensor. It uses narrow bandpass filters, optically eliminating fluorescence produced by the background. Analysis of the results shows that these agree well with those of standard laboratory equipment using a photomultiplier tube (PMT) scanner to detect HSP90 in the nanomolar range. Results from the work show that the used approach is promising for developing a multifunctional, robust, and point-of-care detection system for stress biomarkers.

PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines
Hannover Centre for Optical Technologies (HOT)
Centre of Biomolecular Drug Research (BMWZ)
Conference contribution
No. of pages
Publication date
Publication status
Peer reviewed
ASJC Scopus subject areas
Electronic, Optical and Magnetic Materials, Atomic and Molecular Physics, and Optics, Biomaterials, Radiology Nuclear Medicine and imaging
Electronic version(s)
https://doi.org/10.1117/12.3002242 (Access: Closed)