Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy

authored by
Anatoly Fedorov Kukk, Di Wu, Evelyn Gaffal, Rüdiger Panzer, Steffen Emmert, Bernhard Roth

We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement. The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma. The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements. The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS. The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane. We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.

Hannover Centre for Optical Technologies (HOT)
PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines
External Organisation(s)
Otto-von-Guericke University Magdeburg
Universitätsmedizin Rostock
Journal of Biophotonics
No. of pages
Publication date
Publication status
Peer reviewed
ASJC Scopus subject areas
Chemistry(all), Materials Science(all), Biochemistry, Genetics and Molecular Biology(all), Engineering(all), Physics and Astronomy(all)
Sustainable Development Goals
SDG 3 - Good Health and Well-being
Electronic version(s) (Access: Open)