Bacteria detection in a Kretschmann geometry flow cell at a plasmon-enhanced interface with spectroscopic ellipsometer

authored by

Soraya Zangenehzadeh, Emil Agócs, Harsh Jivani, Lea Könemund, Laurie Neumann, Felix Hirschberg, Svenja Herdan, Rebekka Biedendieck, Dieter Jahn, Bernhard W. Roth, Hans Hermann Johannes, Wolfgang Kowalsky

Abstract

In this work, we investigate whether bacteria can be substantially examined in liquid environment with an ellipsometer in the visible wavelength range. For that investigation a Kretschmann type flow cell was used in which the paths of illuminated and reflected light beams are on the substrate side. To enhance the sensitivity a thin gold layer was applied on the top of glass substrate and the measurement was performed by using surface plasmon polaritons. Porphyrin functionalized coating layers were employed to analyze their performance to capture bacteria and to study the stability and repeatability of the optical response with regard to the ellipsometric measurement. Escherichia coli (E. coli) bacteria were used as first model bacteria and their deposition on the sensing surface was monitored in a real-time measurement in a liquid environment.

Organisation(s)

PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines
Hannover Centre for Optical Technologies (HOT)

External Organisation(s)

Technische Universität Braunschweig

Type

Article

Journal

THIN SOLID FILMS

Volume

764

ISSN

0040-6090

Publication date

01.01.2023

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials, Surfaces and Interfaces, Surfaces, Coatings and Films, Metals and Alloys, Materials Chemistry

Electronic version(s)

https://doi.org/10.1016/j.tsf.2022.139583 (Access: Closed)