Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection

authored by

Lea Könemund, Laurie Neumann, Felix Hirschberg, Rebekka Biedendieck, Dieter Jahn, Hans Hermann Johannes, Wolfgang Kowalsky

Abstract

Traditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.

Organisation(s)

PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines

External Organisation(s)

Technische Universität Braunschweig

Type

Article

Journal

Scientific reports

Volume

12

No. of pages

10

ISSN

2045-2322

Publication date

12.2022

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

General

Electronic version(s)

https://doi.org/10.1038/s41598-022-08272-3 (Access: Open)
https://doi.org/10.1038/s41598-023-46380-w (Access: Open)