SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement
- authored by
- Johanna-Gabriela Walter, Alina Eilers, Lourdes Shanika Malindi Alwis, Bernhard Wilhelm Roth, Kort Bremer
- Abstract
We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10−3 RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.
- Organisation(s)
-
Institute of Technical Chemistry
PhoenixD: Photonics, Optics, and Engineering - Innovation Across Disciplines
Hannover Centre for Optical Technologies (HOT)
- External Organisation(s)
-
Napier University
- Type
- Article
- Journal
- Sensors
- Volume
- 20
- No. of pages
- 11
- ISSN
- 1424-8220
- Publication date
- 20.05.2020
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Analytical Chemistry, Biochemistry, Atomic and Molecular Physics, and Optics, Instrumentation, Electrical and Electronic Engineering
- Electronic version(s)
-
https://doi.org/10.3390/s20102889 (Access:
Open)
