Mueller matrix polarimetry for mouse brain tissue analysis

authored by

Gaurav Sharma, Melbin Siby, Jens Pahnke, Bernhard Roth

Abstract

Mueller Matrix Polarimetry (MMP) has evolved into a prominent area of research, with focus on identifying microstructural changes in bio tissues. This is done by investigating light properties, which is especially useful in early detection of brain cell degradation, among others. We investigated the application of MMP to mouse brain tissue containing amyloid-beta plaques. Alzheimer's disease is widely known for progressing with the production and appearance of the beta plaques. The investigated sample glass slides contained paraffin embedded brain tissue. The tissues were taken at various stages of ageing, i.e. 75, 100, 125, 150, 175, 200, and 225 days. Paraffin tissue blocks were used as an additional sample set for comparison. We set up the experimental Mueller matrix polarimetry to consist of three illuminating laser wavelengths (445 nm, 532 nm, 632 nm). We performed a comparative analysis based on the Mueller matrix elements for each age category and highlighted the importance of certain elements, e.g. m44, for further analysis. We also compared the trends of decomposition parameters and could correlate them with the ageing. Contrary to previous studies, we also report on retardation, diattenuation and polarizance changes. From the higher order statistics, we concluded that mean and standard deviation remained constant across the ages. Skewness values were positive and increased as the age progressed, whereas kurtosis decreased with age. The large available dataset opens the possibility of implementing machine learning methods to assist clinical diagnosis.

Organisation(s)

Hannoversches Zentrum für Optische Technologien (HOT)
PhoenixD: Simulation, Fabrikation und Anwendung optischer Systeme

External Organisation(s)

University of Oslo
Universitätsklinikum Schleswig-Holstein (UKSH)
University of Latvia

Type

Aufsatz in Konferenzband

Publication date

25.01.2025

Publication status

Veröffentlicht

Peer reviewed

Yes

ASJC Scopus Sachgebiete

Elektronische, optische und magnetische Materialien, Atom- und Molekularphysik sowie Optik, Biomaterialien, Radiologie, Nuklearmedizin und Bildgebung

Electronic version(s)

https://doi.org/10.1117/12.3042290 (Access: Geschlossen)