SPR Biosensor Based on Polymer Multi-Mode Optical Waveguide and Nanoparticle Signal Enhancement

verfasst von

Johanna-Gabriela Walter, Alina Eilers, Lourdes Shanika Malindi Alwis, Bernhard Wilhelm Roth, Kort Bremer

Abstract

We present a surface plasmon resonance (SPR) biosensor that is based on a planar-optical multi-mode (MM) polymer waveguide structure applied for the detection of biomolecules in the lower nano-molar (nM) range. The basic sensor shows a sensitivity of 608.6 nm/RIU when exposed to refractive index changes with a measurement resolution of 4.3 × 10⁻³ RIU. By combining the SPR sensor with an aptamer-functionalized, gold-nanoparticle (AuNP)-enhanced sandwich assay, the detection of C-reactive protein (CRP) in a buffer solution was achieved with a response of 0.118 nm/nM. Due to the multi-mode polymer waveguide structure and the simple concept, the reported biosensor is well suited for low-cost disposable lab-on-a-chip applications and can be used with rather simple and economic devices. In particular, the sensor offers the potential for fast and multiplexed detection of several biomarkers on a single integrated platform.

Organisationseinheit(en)

Institut für Technische Chemie
PhoenixD: Simulation, Fabrikation und Anwendung optischer Systeme
Hannoversches Zentrum für Optische Technologien (HOT)

Externe Organisation(en)

Napier University

Typ

Artikel

Journal

Sensors

Band

20

Anzahl der Seiten

11

ISSN

1424-8220

Publikationsdatum

20.05.2020

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Analytische Chemie, Biochemie, Atom- und Molekularphysik sowie Optik, Instrumentierung, Elektrotechnik und Elektronik

Elektronische Version(en)

https://doi.org/10.3390/s20102889 (Zugang: Offen)